BIOGRAPHICAL SKETCH

Academic & Professional Background

• B.S.(Chemistry) 1968 - Illinois Institute of Technology, Chicago
• M.S.(Chemistry) 1970 - Illinois Institute of Technology, Chicago
• Ph.D.(Chemistry) 1972 - Illinois Institute of Technology, Chicago
• M.D.(Medicine) 1975 - Rush Medical College, Chicago, Illinois

Current Research Interests

• Singlet Oxygen Biochemistry and Photochemistry

Selected Publications

Kanofsky JR, Sima PD. Girard's reagent P derivative of Beta-Apo-8'-carotenal: A potent photoprotective agent. Photochemistry and Photobiology 73:349-358, 2001.

Kanofsky JR, Sima PD. Assay for singlet-oxygen generation by plant leaves exposed to ozone. In: Singlet Oxygen, UV-A & Ozone (Methods in Enzymology, Vol 319). Packer L, Sies H (Eds.), Academic Press, Orlando, FL, pp 512-520, 2000.

Kanofsky JR, Sima PD. Reactive absorption of ozone: An assay for reaction rates of ozone with sulfhydryl compounds and with other biological molecules. In: Singlet Oxygen, UV-A & Ozone (Methods in Enzymology, Vol 319). Packer L, Sies H (Eds.), Academic Press, Orlando, FL, pp 505-512, 2000.

Kanofsky JR. Assay for singlet-oxygen generation by peroxidases using 1270-nm chemiluminescence. In: Singlet Oxygen, UV-A & Ozone (Methods in Enzymology, Vol 319). Packer L, Sies H (Eds.), Academic Press, Orlando FL, pp 59-67, 2000.

Kanofsky JR, Sima PD. Structural and environmental requirements for quenching of singlet oxygen by cyanine dyes. Photochem Photobiol 71:361-368, 2000.

Kanofsky JR, Sima PD. Preferential cytotoxicity for multidrug-resistant K562 cells using the combination of a photosensitized and a cyanine dye. J Photochem Photobiol B: Biol 54:136-144, 2000.

Sima PD, Kanofsky JR. Cyanine dyes as protectors of K562 cells from photosensitized cell damage. Photochem Photobiol 71:413-421, 2000.

Rao AVS, Sima PD, Kanofsky JR, Ramasarma T. Inactivation of glucose oxidase by diperoxovanadate-derived oxidants. Arch Biochem Biophys 369:163-173, 1999.

Deadwyler G, Sima PD, Fu Y, Kanofsky JR. Singlet-oxygen mediated inactivation of acetylcholinesterase: A comparison of purified enzyme in solution and enzyme bound to K562 leukemia cells. Photochem Photobiol 65:884-894, 1997.

Fu Y, Sima PD, Kanofsky JR. Singlet-oxygen generation from liposomes: A comparison of 6-cholesterol hydroperoxide formation with predictions from a one-dimensional model of singlet-oxygen diffusion and quenching. Photochem Photobiol 63:468-476, 1996.

Feigenbaum JJ, Choubal MD, Payza K, Kanofsky JR, Crumrine DS. Receptor inactivation by dye-neuropeptide conjugates: 1. The systhesis of cys-containing dye-neuropeptide conjugates. Peptides 17:991-994, 1996.

Feigenbaum JJ, Choubal MD, Crumrine DS, Kanofsky JR. Receptor inactivation by dye-neuropeptide conjugates: 2. Characterization of the quantum yield of singlet oxygen generated by irradiation of dye-neuropeptide conjugates. Peptides 17:1213-1217, 1996.

Feigenbaum JJ, Choubal MD, Crumrine DS, Kanofsky JR, Payza K. Receptor inactivation by dye-neuropeptide conjugates: 3. Comparative binding of dye-neuropeptide conjugates to FMRFamide receptors of Helix aspersa and Loligo pealei. Peptides 17:1279-1284, 1996.

Kanofsky JR, Sima PD. Reactive absorption of ozone by aqueous biomolecule solutions: Implications for the role of sulfhydryl compounds as targets for ozone. Arch Biochem Biopphys 316:52-62, 1995.

Kanofsky JR, Sima PD. Singlet oxygen generation from the reaction of ozone with plant leaves. J Biol Chem 370:7850-7852, 1995.

Fu Y, Kanofsky JR. Singlet-oxygen generation from liposomes: A comparison of time-resolved 1270-nm emission with singlet-oxygen kinetics calculated from a one-dimensional model of singlet-oxygen diffusion and quenching. Photochem Photobiol 62:692-702, 1995.

Kanofsky JR, Baker A, Sima PD. Singlet-oxygen diffusion and quenching in studies of photooxidative damage to intact cells and to cell membranes. In: The Oxygen Paradox. Davies K, Ursini F. (Eds.), CLEUP University Press, Padova, pp. 539-549, 1995.

Kanofsky JR, Sima PD. Singlet-oxygen chemiluminescence at gas-liquid interfaces: Theoretical analysis with a one-dimensional model of singlet-oxygen quenching and diffusion. Arch Biochem Biophys 312:244-259, 1994.

Baker A, Kanofsky JR. Time-resolved studies of singlet-oxygen emission from L1210 leukemia cells labeled with 5-(N-hexadecanoyl) amino eosin. A comparison with one-dimensional model of singlet-oxygen diffusion and quenching. Photochem Photobiol 57:720-727, 1993.

Kanofsky JR, Sima PD. Singlet-oxygen generation at gas-liquid interfaces: A significant artifact in the measurement of singlet-oxygen yields from ozone-biomolecule reactions. Photochem Photobiol 58:335-340, 1993.

Projects Completed or Active (Selected)

• Singlet Oxygen Production from Cell-Membrane-Bound Photosensitizers

Research Funding Sources

• VA Merit Review

Research Methods Employed

• Measurement of Singlet Oxygen Phosphorescence 1268 nm